孙世琪

孙世琪，博士，研究员，博士生导师，农业农村部兽药评审咨询专家，动物疫病净化评估专家，中国农业科学院创新型领军人才。主要从事口蹄疫病毒等重大动物疫病病原致病机理和免疫机理等基础研究以及疫苗和诊断等应用研究。先后主持国家重点研发计划课题、国家自然科学基金面上项目、国家国际科技合作、教育部留学回国人员科研启动基金、甘肃省科技重大专项、甘肃省国际科技合作等项目。获一类兽药注册证书2项（排名1,2），中国专利优秀奖（排名第1），甘肃省发明专利一等奖（排名第1），大北农科技奖（排名第1），中国农业科学院成果转化奖-知识产权转化奖（排名第1），中国农业科学院科学技术成果奖-杰出科技创新奖（第2），中国农业科学院-大北农人才奖。主编著作1部，参编著作5部，参加翻译著作2部，获国际发明专利4项，国家发明专利20多项，以第一或通讯作者发表SCI论文30余篇；培养研究生20余名。

一、主持项目：

1. 国家重点研发计划课题，FMDV隐性感染的形成与传播机制（2021YFD1800303）。

2. 国家自然科学基金面上项目“热休克蛋白HSP60参与口蹄疫病毒形态发生的作用及其机制（32072859）”。

3. 国家国际科技合作专项项目“鸡传染性支气管炎病毒宿主嗜性及新型细胞苗的合作研究（2014DFA31890）”。

4. 国家外国专家局引进国外技术、管理人才项目“鸡传染性支气管炎病毒细胞工程疫苗株的构建和免疫特性研究（20156200061）”。

5. 甘肃省科技重大专项“口蹄疫和猪圆环病病毒样颗粒疫苗的创制（1102NKDA034）”。

6. 甘肃省国际科技合作计划“鸡传染性支气管炎新型重组苗的创制（1104WCGA185）”。

7. 教育部留学回国人员科研启动基金“猪瘟病毒P7蛋白的功能研究”。

二、获奖

1. 口蹄疫病毒样颗粒及制备方法和用途 中国专利优秀奖. 2022.7

2. 口蹄疫病毒样颗粒及制备方法和用途. 甘肃省专利一等奖，甘肃省人民政府，2020.1.11

3. 口蹄疫病毒样颗粒及制备方法和用途，中国农业科学院成果转化奖-知识产权转化奖，2020.12

4. 病毒样颗粒关键技术平台及疫苗创制 中国农业科学院科技成果奖（杰出科技创新奖）2023.1.2

5. 中国农业科学院-大北农人才奖（杰出人才奖）。中国农业科学院，2023.1.12

6. 原核表达系统获得病毒样颗粒关键技术及平台建立.大北农科技奖创意奖。北京大北农科技集团股份有限公司，2015.11

三、产品研发

1. 一类新兽药：猪口蹄疫O型病毒样颗粒疫苗，（2021）新兽药证字58号

2. 一类新兽药：牛口蹄疫O型病毒样颗粒疫苗，（2021）新兽药证字57号

四、代表专利：

1. Shiqi Sun, Huichen Guo, et al. Method for Preparing Foot-And-Mouth Disease Virus-Like Particles. US10829741B2. 2020.11.10

2. Huichen Guo, Shiqi Sun, et al.Virus-Like Particle of Senecavirus A. US010532077B2. 2020.1.14

3. Huichen Guo, Shiqi Sun, et al. Mineralized Foot-And-Mouth Disease Virus Like Particles, And Preparation Method And Use. US010532092B2. 2020.1.14

4. GUO, Huichen; SUN, Shiqi; et al. Virus-Like Particle(VLP), and Preparation Method and Use Thereof. 2022 09860, 2022.11.30

5. 郭慧琛;孙世琪等.一种口蹄疫病毒VP1嵌合纳米颗粒及其制备方法和应用.ZL 2022 1 0771918.0, 20230509

6. 郭慧琛;孙世琪等. 一种耐热型口蹄疫重组病毒株及其在制备口蹄疫灭活疫苗中的应用. ZL202010724770.6, 20221025

7. 郭慧琛;孙世琪等. 一种耐热型口蹄疫重组病毒株、由该病毒株制成的灭活疫苗及其应用. ZL202010725839.7, 20220503.

8. 孙世琪，白满元等。一种基于病毒样颗粒的C 型口蹄疫病毒抗体竞争ELISA试剂盒。ZL201811492145.2，20210803

9. 郭慧琛, 孙世琪等. 一种口蹄疫病毒样颗粒体外组装的方法及其应用。 ZL202010116531.2, 20210730

10. 郭慧琛，孙世琪等。用于O型口蹄疫病毒抗体定量检测的上转换发光免疫层析试纸条及其制备方法ZL202010061291.0，20210601

11. 郭慧琛，孙世琪等. 金纳米颗粒作为佐剂在制备病毒样颗粒疫苗中的应用. ZL201710553111.9，20201110

12. 郭慧琛，孙世琪等. 矿化口蹄疫病毒样颗粒及其制备方法和应用. ZL201710539048.3, 20201016

13. 郭慧琛，孙世琪等。口蹄疫病毒样颗粒在作为重组质粒运载载体中的应用。ZL201610929291.1, 20200703

14. 孙世琪, 郭慧琛. 一种O型口蹄疫病毒样颗粒及其制备方法和用途. ZL201610929279.0,20190702

15. 郭慧琛，孙世琪等. 一种A型塞尼卡病毒病毒样颗粒及其制备方法和用途. ZL201810483236.3 20191224

16. 孙世琪, 郭慧琛等. 一种肿瘤药物靶向载体及应用. ZL201510128809.7，20180706

17. 孙世琪, 郭慧琛等.一种用病毒样颗粒包被量子点的方法. ZL201510037803.9, 20170922

18. 孙世琪，张韵等. 猪圆环病毒2型抗体快速检测层析试纸条及制备方法. ZL201610190933.0,20170919

19. 郭慧琛,孙世琪等.嵌合IBV 4/91株纤突蛋白膜外区基因片段的重组传染性支气管炎病毒及其构建方法和应用.ZL201310573277.9 20160127

20. 郭慧琛;孙世琪等. 犬细小病毒病毒样颗粒的制备及用途. 专利号：ZL 201310381323.5，20151223

21. 郭慧琛, 孙世琪等. 一种运载猪圆环病毒抗原表位蛋白的纳米载体及应用. ZL201110288387.1, 20150902

22. 郭慧琛, 孙世琪等. 适于细胞培养的嵌合IBV H120 S1基因膜外区的重组病毒及其构建方法和应用. ZL201310196897.5, 20150318

23. 孙世琪, 郭慧琛等. 口蹄疫病毒样颗粒及制备方法和用途. 中华人民共和国国家知识产权局, ZL201010251915.1, 20121010

24. 刘湘涛，孙世琪等. 一种检测猪瘟病毒的方法. 中华人民共和国国家知识产权局. 专利号：ZL200310103412X, 2006.02.28.

五、代表著作和文章（*通讯作者）

1. 郭慧琛 孙世琪 主编，动物病毒样颗粒技术. ISBN 978-7-03-073385-6 中国科学出版社. 2022.11

2. Liu H, Teng Z, Dong H, Li J, Waheed Abdullah S, Zhang Y, Wu J, Guo H, Sun S*. Poly(I:C) and CpG improve the assembly of foot-and-mouth disease virus-like particles and immune response in mice. Virology. 2023 Feb;579:94-100.

3. Abdullah SW, Wu J, Wang X, Guo H, Sun S*. Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses. mBio. 2023 Apr 25;14(2):e0035823.

4. Wang X, Abdullah SW, Wu J, Tang J, Zhang Y, Dong H, Bai M, Wei S, Sun S*, Guo H*. Foot-and-mouth disease virus downregulates vacuolar protein sorting 28 to promote viral replication. J Virol. 2023 Aug 31;97(8):e0018123.

5. Dong H, Liu P, Bai M, Wang K, Feng R, Zhu D, Sun Y, Mu S, Li H, Harmsen M, Sun S*, Wang X*, Guo H* Structural and molecular basis for foot-and mouth disease virus neutralization by two potent protective antibodies. Protein Cell. 2022 Jun;13(6):446-453.

6. Tang J, Abdullah SW, Li P, Wu J, Pei C, Mu S, Wang Y, Sun S*, Guo H*. Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C. mBio. 2022 Oct 26;13(5):e0143422.  

7. Hou F, Teng Z, Ru J, Liu H, Li J, Zhang Y, Sun S*, Guo H*. Flower-like mesoporous silica nanoparticles as an antigen delivery platform to promote systemic immune response. Nanomedicine NBM. 2022 Feb 16;42:102541.

8. Teng Z, Hou F, Bai M, Li J, Wang J, Wu J, Ru J, Ren M, Sun S*, Guo H*. Bio-mineralization of virus-like particles by metal-organic framework nanoparticles enhances the thermostability and immune responses of the vaccines. J Mater Chem B. 2022, Apr 13;10(15):2853-2864.  

9. Yang K, Song H, Shi X, Ru J, Tan S, Teng Z, Dong H, Guo H, Wei F*, Sun S*. Preparation of a polysaccharide adjuvant and its application in the production of a foot-and-mouth disease virus-like particles vaccine. Biochemical Engineering Journal, 2022,184:108479.

10. Teng Z#, Sun S#, Luo X, Zhang Z, Seo H, Xu X, Huang J, Dong H, Mu S, Du P, Zhang Z*, Guo H*. Bi-functional gold nanocages enhance specific immunological responses of foot-and-mouth disease virus-like particles vaccine as a carrier and adjuvant. Nanomedicine. 2021 Jan 20;33:102358.

11. Dong H, Lu Y, Zhang Y, Mu S, Wang N, Du P, Zhi X, Wen X, Wang X, Sun S*, Zhang Y*, Guo H*. A heat-induced Mutation on VP1 of FMDV Serotype O Enhanced Capsid Stability and the Immunogenicity. J Virol. 2021 Jul 26;95(16):e0017721.

12. Han S, Wang X, Guan J, Wu J, Zhang Y, Li P, Liu Z, Abdullah SW, Zhang Z, Jin Y, Sun S*, Guo H*. Nucleolin Promotes IRES-Driven Translation of Foot-and-Mouth Disease Virus by Supporting the Assembly of Translation Initiation Complexes. J Virol. 2021 Jun 10;95(13):e0023821.

13. Abdullah SW, Wu J, Zhang Y, Bai M, Guan J, Liu X, Sun S*, Guo H*. DDX21, a HostRestriction Factor of FMDV IRES-Dependent Translation and Replication. Viruses 2021, 13, 1765.

14. Yang Y, Teng Z, Lu Y, Luo X, Mu S, Ru J, Zhao X, Guo H, Ran X, Wen X*, Sun S*. Enhanced immunogenicity of foot and mouth disease DNA vaccine delivered by PLGA nanoparticles combined with cytokine adjuvants. Res Vet Sci. 2021 Feb 10;136:89-96.

15. Abdullah SW, Han S, Wu J, Zhang Y, Bai M, Jin Y, Zhi X, Guan J, Sun S*, Guo H*. The DDX23 Negatively Regulates Translation and Replication of Foot-and-Mouth Disease Virus and Is Degraded by 3C Proteinase. Viruses 2020, 12(12), 1348

16. Lu Y, Dong H, Li J, Li L, Wang M, Liu H, Teng Z, Zhang Y, Jin Y, Guo H, Yang Y, Wen X*, Sun S*. Enhanced protective immune response of foot-and-mouth disease vaccine through DNA-loaded virus-like particles. Microb Pathog. 2020;143:104130. 

17. Mu S, Abdullah SW, Zhang Y, Han S, Guo H, Li M, Dong H, Xu J, Teng Z, Wen X, Sun S*. Development of a novel SYBR green I-based quantitative RT-PCR assay for Senecavirus A detection in clinical samples of pigs. Mol Cell Probes. 2020 Aug 5:101643.

18. Ran X#, Yang Z#, Bai M, Zhang Y, Wen X, Guo H, Sun S*. Development and validation of a competitive ELISA based on bacterium-original virus-like particles of serotype O foot-and-mouth disease virus for detecting serum antibodies. Appl Microbiol Biotechnol. 2019,103(7):3015-3024.

19. Teng Z#, Sun S#, Chen H, Huang J, Du P, Dong H, Xu X, Mu S, Zhang Z, Guo H. Golden-star nanoparticles as adjuvant effectively promotes immune response to foot-and -mouthdisease virus-like particles vaccine. Vaccine,2018, 36: 6752–6760

20. Wei Y, Ji Y, Guo H, Zhi X, Han S, Zhang Y, Gao Y, Chang Y, Yan D, Li K, Liu DX*, Sun S*. CD59 association with infectious bronchitis virus particles protects against antibody-dependent complement-mediated lysis. J Gen Virol. 2017,98: 2725–2730.

21. Fan X, Han S, Yan D, Gao Y, Wei Y, Liu X, Liao Y, Guo H*, Sun S*. Foot-and-mouth disease virus infection suppresses autophagy and NF-кB antiviral responses via degradation of ATG5-ATG12 by 3Cpro. Cell Death Dis. 2017 Jan 19;8(1):e2561.

22. Han SC¹, Guo HC¹, Sun SQ*, Jin Y, Wei YQ, Feng X,Yao XP, Cao SZ, Liu DX, Liu XT. Productive Entry of Foot-and-Mouth Disease Virus via Macropinocytosis Independent of Phosphatidylinositol 3-Kinase. Sci Rep. 2016 Jan 13;6:19294.

23. Ma Y, Wang H, Yan D, Wei Y, Cao Y, Yi P, Zhang H, Deng Z, Dai J, Liu X, Luo J, Zhang Z*, Sun S*, Guo H*. Magnetic Resonance Imaging Revealed Splenic Targeting of Canine Parvovirus Capsid Protein VP2. Sci Rep. 2016 Mar 21;6:23392.

24. Guo H, Zhu J, Tan Y, Li C, Chen Z, Sun S*, Liu G*. Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits. Antiviral Res. 2016 Apr 23;131:85-91.

25. Yan D, Wei YQ, Guo HC, Sun SQ*. The application of virus-like particles as vaccines and biological vehicles. Appl Microbiol Biotechnol. 2015 Dec; 99(24): 10415-32.

26. Guo HC, Jin Y, Zhi XY, Yan D, Sun SQ*. NLRP3 Inflammasome Activation by Viroporins of Animal Viruses. Viruses. 2015 Jun 24;7(7):3380-91.

27. Han SC, Guo HC, Sun SQ*, Shu L, Wei YQ, Sun H, Cao SZ, Peng GN, Liu XT. Full-length genomic characterizations of two canine parvoviruses prevalent in Northwest China. Arch Microbiol. 2015 May; 197(4):621-6.

28. Xu J, Guo HC, Wei YQ, Shu L, Wang J, Li JS, Cao SZ, Sun SQ*. Phylogenetic Analysis of Canine Parvovirus Isolates from Sichuan and Gansu Provinces of China in 2011. Transbound Emerg Dis. 2015 Feb;62(1):91-5.

29. Dong H, Guo HC, Sun SQ*. Virus-like particles in picornavirus vaccine development. Appl Microbiol Biotechnol. 2014 May;98(10):4321-9

30. Xu J, Guo HC, Wei YQ, Dong H, Han SC, Ao D, Sun DH, Wang HM, Cao SZ, Sun SQ*. Self-assembly of virus-like particles of canine parvovirus capsid protein expressed from Escherichia coli and application as virus-like particle vaccine. Appl Microbiol Biotechnol. 2014 Apr;98(8):3529-38

31. Wei YQ, Guo HC, Dong H, Wang HM, Xu J, Sun DH, Fang SG, Cai XP, Liu DX, Sun SQ*. Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain. Appl Microbiol Biotechnol. 2014 Feb;98(4):1727-35.

32. Guo HC, Sun SQ*, Jin Y, Yang SL, Yin SH, Sun DH, Wei YQ, Ma JW, Liu ZX, Guo JH, LuoJX, Yin H, Liu XT, Liu DX*. Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle. Vet Res. 2013 Jul 4;44:48.

33. Guo HC, Sun SQ*, Sun DH, Wei YQ, Xu J, Huang M, Liu XT, Liu ZX, Luo JX, Yin H, Liu DX*. Viroporin Activity and Membrane Topology of Classic Swine Fever Virus p7 Protein. Int J Biochem Cell Biol. 2013 Jul;45(7):1186-1194.

34. Sun SQ, Yin SH, Guo HC*, Jin Y, Shang YJ, Liu XT*. Genetic Typing of Classical Swine Fever Virus Isolates from China. Transbound Emerg Dis. 2013 Aug;60(4):370-5.

35. Guo HC, Sun SQ*. Lanthanide doped upconverting phosphors for bioassay and therapy. Nanoscale. 2012 Nov 7;4(21):6692-706.

36. Guo H, Hao R, Qian H, Sun S*, Sun D, Yin H, Liu Z, Liu X*. Upconversion nanoparticles modified with aminosilanes as carriers of DNA vaccine for foot-and-mouth disease. Appl Microbiol Biotechnol. 2012 Sep;95(5):1253-63.

37. Sun SQ, Guo HC*, Sun DH, Yin SH, Shang YJ, Cai XP, Liu XT*. Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2. Virol J. 2010; 7: 274.(IF:2.44)

38. Sun SQ, Liu XT, Guo HC, Yin SH, Shang YJ, Feng X, Liu ZX, Xie QG. Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus. 2007. J Gen Virol. 88, 842–848 (IF:3.092)