闫鸿斌

一、个人简介

闫鸿斌，博士，副研究员，硕士研究生导师，美国乔治华盛顿大学博士后。2003年7月到中国农业科学院兰州兽医研究所参加工作至今，长期以来主要从事棘球蚴的发育与致病机理研究和诊断技术与疫苗等防控技术研究。近年来，先后主持国家自然科学基金项目（1项）、十三五和十四五重点研发项目子课题（2项）、甘肃省联合基金重点项目1项、中国农业科学院基本科研业务费项目（1项）等，执行主持中国农业科学院国际农业科学计划项目1项，参加国家、省部级各类科研项目多项；共发表科技论文90多篇，其中以第一作者或通讯作者（含并列通讯作者）发表SCI论文40余篇；（副）主编著作一部《国家动物疫病防控专著系列-棘球蚴病》；参与修订农业行业标准1项；获得授权国家发明专利9项；获得甘肃省自然科学奖（三等）和科技进步奖（三等）各1项。

二、 联系方式：

1．研究方向：人兽共患病与兽医公共卫生学

2．邮箱：yanhongbin@caas.cn

3．所在单位：中国农业科学院兰州兽医研究所

4．通讯地址：甘肃省兰州市盐场堡徐家坪1号

三、教育经历

2010.09 – 2013.06，中国农业科学院研究生院，预防兽医学专业，博士

2005.09 – 2007.06，中国农业科学院研究生院，预防兽医学专业，硕士

1999.09 – 2003.06，甘肃农业大学，动物医学专业，学士

四、工作经历

2016.1 - 至今 中国农业科学院兰州兽医研究所，副研究员

2008.1 - 2015.12中国农业科学院兰州兽医研究所，助理研究员

2003.08至2007.12, 中国农业科学院兰州兽医研究所,研究实习员

2013.7-2014.12 美国乔治华盛顿大学（George Washington University），博士后

五、承担项目

1．主持国家自然基金青年科学基金项目：利用RNA引导的CRISPR/Cas9系统研究多房棘球绦虫丝氨酸蛋白酶抑制剂的功能（项目编号：31402191，2015.01-2017.12），25万元。

2．主持“十四五”国家重点研发计划项目子课题：病原学与防疫技术体系研究-人兽共患病流行病学数据库、传播和演化规律的建立（课题一）（项目编号：2022YFC2304001，2022.12-2025.11），100万元。

3．主持“十三五”国家重点研发计划项目子课题：畜禽重要人兽共患寄生虫病源头防控与阻断技术研究（项目编号：2017YFD0501300，2017.07-2021.06），40万元。

4．主持甘肃省联合科研基金重点项目：中性粒细胞胞外诱捕网在多房棘球蚴感染及包囊形成中的作用机制（项目编号：24JRRA807，2024.7-2026.6），50万元。

5．执行主持中国农业科学院国际农业科学计划项目：中亚地区重要人兽共患病和新发再发重大动物应疫病流行病学研究与防控技术推广应用（项目编号：CAASTIP-2024-05，2024.1-2028.12)，2000万元。

6．主持中国农业科学院中央级科研院所基本科研业务费：利用CRISPR/Cas9技术研究Wnt信号通路在多房棘球蚴发育过程中的功能（项目编号：161031201 6012，2016.01-2019.12），69万元。

7．主持甘肃省科技重大专项子课题：牛、羊包虫病疫苗研究（项目编号：23ZDNA007、24ZD13NA008，2024.01-2027.12），64万元。

六、代表论文与著作

科技论文：

以第一作者（含并列第一）或通讯作者（含并列通讯）发表的SCI收录论文40余篇，代表作如下：

1．Ou Z, Li L, Ren P, Zhou TT, He F, Chen J, Cai H, Han X, Wu YD, Li J, Li XR, Tan Q, Li W, Chen Q, Zhang N Z, He X, Chen WG, Zhao Y, Sun J, Zhang Q, Wu YT, Liang Y, You J, Hu G, Tian XQ, Liao S, Fu BQ, Chen A, Cai X P, Yang H, Wang J, Jin X, Xu X, Jia WZ*, Li J*, Yan HB*. Spatiotemporal Transcriptomic Profi��������ling Reveals the Dynamic Immunological Landscape of Alveolar Echinococcosis. Advanced Science, 2025, 2405914.

2．Shahbaz Ul Haq, Muhammad Abdullah Malik, Ayed Alshammari , Abu Bakar Yameen, Majed H Wakid, Mughees Aizaz Alvi, Abdulbaset Mohammad Kabli, Muhammad Saqib, Warda Qamar, Muhammad Sohail Sajid, Fenfei Gao, Li Li, Fu BQ, Yan HB*, Jia WZ*Genetic variation and population structure of Taenia multiceps (Coenurus cerebralis) based on mitochondrial cox1 gene: A comprehensive global analysis. Infect Genet Evol, 2024, 125:105676.

3．Cai S, Wang C, Tian F, Liu M, Yan HB*, Xu Z*, Qu G*. Dual-RPA assay for rapid detection and differentiation of E.granulosus and E. multilocularis. Microb Pathog, 2024, 189:106600.

4．Alvi MA, Ali RMA, Li L, Saqib M, Qamar W, Hassan A, Ghafoor M, Rahman SU, Khan MUZ, Fu BQ, Yin H, Yan HB*, Jia WZ*. Phylogeny and population structure of Echinococcus granulosus (sensu stricto) based on full-length cytb-nad2-atp6 mitochondrial genes - First report from Sialkot District of Pakistan. Mol Biochem Parasitol, 2023, 253: 111542..

5．Alvi MA, Ali RMA, Haq SU, Ayed A, Rizwan Bashir R, Li L, Saqib M, MS, Ghafoor, M, Imran M, Ijaz MU, Fu BQ, Yan HB*, Jia WZ*. Revealing novel cytb and nad5 genes-based population diversity and benzimidazole resistance in Echinococcus granulosus isolated bovines ������ ������ ������ ������ ������ ������ ������ ������ ������ ������ ������ ������ ������ Front Vet Sci, 2023, 10: 1191271.

6．Shumuye NA, Li L, Ohiolei JA, Qurishi SA, Li WH, Zhang NZ, Wu YT, Wu YD, Gao SZ, Zhang FH, Tian XQ, Tian WJ, Fu Y, Wang XZ, Pan YH, Zhan F, Zhang LS, Guo MK, Li WD, Fu BQ, Yan HB*, Jia WZ*. Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences. Parasitol Res, 2023, 122(5): 1107-1126.

7．Alvi MA, Khalid A, Ali RMA, Saqib M, Qamar W, Li L, Ahmad B, Fu BQ, Yan HB*, Jia WZ*. Genetic variation and population structure of Fasciola hepatica: an in silico analysis. Parasitol Res, 2023, 122(9): 2155-2173.

8．Alvi MA, Ali RMA, Khan S, Saqib M, Qamar W, Li L, Fu BQ, Yan HB*, Jia WZ*. Past and present of diagnosis of echinococcosis: A review (1999-2021). Acta Trop, 243: 106925.

9．Shumuye NA, Li L, Ohiolei JA, Li WH, Zhang NZ, Wu YT, Wu YD, Tian WJ, Zhang LS, Nian XF, Dai GD, Chen WG, Gao SZ, Tian XQ, Liu JS, Li B, Kebede N, Fu BQ, Yan HB*, Jia WZ*. Domestic Echinococcus multilocularis transmission: evidence from mitochondrial DNA analysis. Infect Dis Poverty, 2023, 12(1): 72.

10．Nian XF, Li L, Ma XS, Li XR, Li WH, Zhang NZ, Ohiolei JA, Li L, Dai GD, Liu YH, Yan HB*, Fu BQ, Xiao S*, Jia WZ*. Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. PLoS Negl Trop Dis, 2022, 16(5): e0010435.

11．Wu YD, Dai GD, Li L, Littlewood DTJ, Ohiolei JA, Zhang LS, Guo AM, Wu YT, Ni XW, Shumuye NA, Li WH, Zhang NZ, Fu BQ, Fu Y, Yan HB*, Jia WZ*. Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the "Out of Qinghai-Tibet Plateau* Hypothesis of Cyclophyllideans. Front Microbiol 2022, 13:747484.

12．Dai GD, Yan HB, Li L, Zhang LS, Liu ZL, Gao SZ, Ohiolei JA, Wu YD, Guo AM, Fu BQ, Jia WZ*. Molecular Characterization of a New Moniliformis sp. From a Plateau Zokor (Eospalax fontanierii baileyi) in China. Front Microbiol 2022, 13:806882.

13．Ohiolei JA, Yan HB, Odeniran PO, Li L, Shumuye NA, Qurishi SA, Isaac C, Fu BQ, Jia WZ*. Echinococcus granulosus sensu lato in animal intermediate hosts: What is with the organ location? Vet Parasitol 2022, 304:109695.

14．Yan HB*, Li L, Li WH, Zhu GQ, Li JQ, Wu YT, Zhang NZ, Wu YD, Li M, Zhang LS, Yao G, Tian WJ, Li L, Li WJ, Guo AM, Dai GD, Fu BQ, Ohiolei JA*, Jia WZ*. Echinococcus shiquicus in Qinghai-Tibet plateau: population structure and confirmation of additional endemic areas. Parasitology, 2021, 148: 879-886.

15．Ohiolei JA, Yan HB*, Li L, Alvi MA, Muku RJ, WuYD, Zhang NZ, Li WH, Guo AM, Wang XL, Fu BQ, Jia WZ*. PCR-RFLP assay confirms the existence of different mitochondrial lineages of Taenia hydatigena including a possible geographically restricted group, 2021, Transbound Emerg Dis, 2021, 00: 1–8. (IF=5.005)

16．Ohiolei JA, Yan HB, Li L, Li WH, Wu YD, Alvi MA, Zhang NZ, Fu BQ, Wang XL, Jia WZ*. (2021) A new molecular nomenclature for Taenia hydatigena: mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions. Parasitology, 148(3): 311-326.

17．Wu YD, Li L, Fan YL, Ohiolei JA, Ni XW, Li WH, Li JQ, Zhang NZ, Fu BQ, Yan HB*, Jia WZ*. Genetic Evolution and Implications of the Mitochondrial Genomes of Two Newly Identified Taenia spp. in Rodents from Qinghai-Tibet Plateau. Front Microbiol, 2021, 12: 647119. (IF=5.640)

18．Alvi MA, Li L, Ohiolei JA, Qamar W, Saqib M, Tayyab MH, Altaf J, Ashfaq K, Hassan A, Jamal M, Wahab A, Alvi AA, Usman M, Bajwa MRK, Fu BQ, Yan HB*, Jia WZ*. Hydatigera taeniaeformis in urban rats (Rattus rattus) in Faisalabad, Pakistan. Infection, Genetics and Evolution, 2021, 92: 104873.

19．Alvi MA, Li L, Saqib M, Ohiolei JA, Tayyab MH, Tahir MM, Qamar W, Altaf W, Usman M, Hassan A, Bajwa MRK, Fu B-Q, Yan HB*, Jia WZ*. Copro-Antigenic Sandwich ELISA based Epidemiological Survey on Prevalence of Echinococcus granulosus Infection in Dogs: First Insight from Pakistan. International Journal of Agriculture and Biology, 2021, 25: 881‒��������887

20．Alvi MA, Li L, SaqibM, Ohioleib JA; Younas MW, Tayyab MH, Hassan A, Qamar W, Alvi AAFu BQ, Yan HB*, Jia WZ*. Serologic evidence of Echinococcus granulosus in slaughterhouses in Pakistan: global alarm for butchers in developing countries. Journal of Infection in Developing Countries, 2021, 15(6):861-869.

21．Alvi MA, Ohiolei JA, Saqib M, Li L, Tayyab MH, Alvi AA, Wu YT, Fu BQ, Yan HB*, Jia WZ*. Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA. Parasit Vectors, 2020, 13(1): 347.

22．Alvi MA, Ohiolei JA, Saqib M, Li L, Muhammad N, Tayyab MH, Qamar W, Alvi AA, Wu YD, Li XR, Fu BQ, Yan HB*, Jia WZ*. Preliminary information on the prevalence and molecular description of Taenia hydatigena isolates in Pakistan based on mitochondrial cox1 gene. Infect Genet Evol, 2020, 84: 104481.

23．Alvi MA, Ohiolei JA, Saqib M, Tayyab MH, Zafar Khan MU, Li Li, Aqib AI, Hassan A, Alvi AA, Qamar W, Fu BQ, Yan HB*, Jia WZ*. First report on molecular characterization of Taenia multiceps isolates from sheep and goats in Faisalabad, Pakistan. Front Vet Sci, 2020, 7: 594599.

24．Zhu GQ, Yan HB, Li Li, Ohiolei JA, Wu YT, Li WH, Zhang NZ, Fu BQ, Jia WZ. First report on the phylogenetic relationship, genetic variation of Echinococcus shiquicus isolates in Tibet Autonomous Region, China. Parasit Vectors. 2020, 13(1): 590

25．Ohiolei JA, Xia CY, Li L, Liu JZ, Tang WQ, Wu YT, Danqulamu, Zhu GQ, Shi B, Fu BQ, Yin H, Yan HB*, Jia WZ*. Genetic variation of Echinococcus spp. in yaks and sheep in the Tibet Autonomous Region of China based on mitochondrial DNA. Parasit Vectors, 2019, 27, 12(1): 608.

26．Zhu GQ, Li L, Ohiolei JA, Wu YT, Li WH, Zhang NZ, Fu BQ, Yan HB*, Jia WZ*. A multiplex PCR assay for the simultaneous detection of Taenia hydatigena, T. multiceps, T. pisiformis, and Dipylidium caninum infections. BMC Infect Dis, 2019, 19(1): 854.

27．Yan HB, Smout MJ, Ju C, Folley AE, Skinner DE, Mann VH, Loukas A, Hu W, Brindley PJ, Rinaldi G*. Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes. Antimicrob Agents Chemother, 2018, 62(8):e02568-02517.

28．Li JQ, Li L, Fan YL, Fu BQ, Zhu XQ, Yan HB*, Jia WZ*. Genetic diversity in Echinococcus multilocularis from the plateau vole and plateau pika in Jiuzhi County, Qinghai Province, China. Front Microbiol, 2018, 9:2632

29．Wu Y, Li L, Zhu G, Li W, Zhang N, Li S, Yao G, Tian W, Fu B, Yan HB*, Jia WZ*. Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau. Parasit Vectors, 2018, 11(1):166.

30．Rinaldi G, Yan HB, Nacif-Pimenta R, Matchimakul P, Bridger J, Mann VH, Smout MJ, Brindley PJ, Knight M*. Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni. Int J Parasitol, 2015, 45(8):527-535.

31．Yan HB, Lou ZZ, Li L, Brindley PJ, Zheng YD, Luo XN, Hou JL, Guo AJ, Jia WZ*, Cai XP*. Genome-wide analysis of regulatory proteases sequences identified through bioinformatics data mining in Taenia solium. BMC genomics, 2014, 15:428.

32．Liu GH†��������, Yan HB†��������, Otranto D, Wang XY, Zhao GH, Jia WZ*, Zhu XQ*. Dicrocoelium chinensis and Dicrocoelium dendriticum (Trematoda: Digenea) are distinct lancet fluke species based on mitochondrial and nuclear ribosomal DNA sequences. Mol Phylogenet Evol, 2014, 79:325-331.

33．Yan HB, Wang XY, Lou ZZ, Li L, Blair D, Yin H, Cai JZ, Dai XL, Lei MT, Zhu XQ, Cai XP*, Jia WZ*. The mitochondrial genome of Paramphistomum cervi (Digenea), the first representative for the family Paramphistomidae. PLoS One, 2013, 8(8):e71300.

34．Yan HB, Lou Z, Li L, Ni X, Guo A, Li H, Zheng Y, Dyachenko V, Jia WZ*. The nuclear 18S ribosomal RNA gene as a source of phylogenetic information in the genus Taenia. Parasitol Res, 2013, 112(3):1343-1347.

35．Yan H, Bo X, Liu Y, Lou Z, Ni X, Shi W, Zhan F, Ooi H, Jia WZ*. Differential diagnosis of Moniezia benedeni and M. expansa (Anoplocephalidae) by PCR using markers in small ribosomal DNA (18S rDNA). Acta Vet Hung, 2013, 61(4):463-472.

36．贾万忠, 闫鸿斌（副主编）, 李立, 娄忠子. 棘球蚴病. 中国农业出版社, 北京, 2015. 690千字.（专著）

七、专利和标准

1．闫鸿斌, 李立, 朱国强, 娄忠子, 李双男, 李文卉, 张念章, 贾万忠, 付宝权. 用于检测和区分多种以犬为终末宿主的常见大中型绦虫的检测试剂盒. ZL201710013354.3.

2．贾万忠, 娄忠子, 李宏民, 闫鸿斌, 倪兴维. 截短细粒棘球蚴EG95重组抗原在毕赤酵母中的高效表达方法. 专利号ZL201210103512.1.

3．贾万忠, 李立, 陈冰, 肖志峰, 戴建武, 闫鸿斌, 娄忠子. 一种棘球蚴体外三维培养模型及其应用. 专利号ZL201510212551.9.

4．娄忠子, 贾万忠, 闫鸿斌, 李宏民, 李立, 范彦雷, 倪兴维. 用于鉴定和鉴别棘球绦虫的检测试剂盒. 专利号ZL201310102181.4.

5．贾万忠, 倪兴维, 闫鸿斌, 娄忠子, 李宏民, 李立. 从犬粪便中检测石渠棘球绦虫（蚴）病原的试剂盒. 专利号ZL201310342870.2.

6．贾万忠, 倪兴维, 闫鸿斌, 娄忠子, 李宏民. 从犬粪便中检测多房棘球绦虫病原的试剂盒. 专利号ZL201110346474.8.

7．闫鸿斌, 李立, 贾万忠, 李文卉, 娄忠子. 双层实验犬笼. 专利号ZL201620126341.8.

8．贾万忠, 闫鸿斌, 李立, 娄忠子, 付宝权, 李有全, 殷宏. 动物棘球蚴病诊断技术（NY/T1466-2018）.（农业行业标准）

八、获得奖励

1．朱兴全, 刘国华, 贾万忠, 林瑞庆, 闫鸿斌. 动物重要寄生虫线粒体基因组解析及系统发育研究. 甘肃省自然科学奖三等奖（2018-Z3-008）, 2019.01.

2．吴志仓，张俊文，史万贵，周绪正，闫鸿斌，林学仕，陈金苹. 羊寄生虫病综合防控技术体系建立与示范. 甘肃省科技进步三等奖（2018-J3-067）, 2019.01.